Journal: bioRxiv
Article Title: Monocyte-derived macrophages aggravate pulmonary vasculitis via cGAS/STING/IFN-mediated nucleic acid sensing
doi: 10.1101/2022.05.30.493983
Figure Lengend Snippet: (A) Schematic of ANCA-associated pulmonary vasculitis (AAPV) induction. Anti MPO, monoclonal antibodies 6D1 and 6G4 (B) H&E stainings of lung cryosections 3d after disease induction from the indicated mouse groups. Bar = 500 µm. (C, D) Lung injury score (0-4) quantified from H&E lung cryosections. (E-H) Representative photographs (E) and quantification (F-H) of pulmonary hemorrhages in BALF. Treatments and time points as indicated. Hb, hemoglobin. (I) Light-sheet fluorescence microscope images from clarified left lungs isolated from mice treated as indicated and injected with Texas red-labeled albumin. Shown is a representative of two mice. (J-L) Kinetics of weight loss (J), SpO 2 (%) in peripheral blood (K), and survival (L) of mice as indicated in (A). (M-O) Flow cytometric quantification (M, N) and UMAP dimensionality reduction plots (O) of immune cells in BALF. Cell identity gates are shown in Fig. S2. Each symbol represents an individual mouse. Shown is mean ± S.E.M. Shown is a representative of at least three independent experiments with n=5 mice/group unless otherwise indicated. *, P<0.05; **, P<0.01; ***, P<0.001. AAPV, ANCA-associated pulmonary vasculitis; BALF, bronchoalveolar lavage fluid; OD, OD 400 – OD 600 .
Article Snippet: Dimensionality reduction of flow cytometric data was performed using the Uniform Manifold Approximation and Projection for Dimension Reduction (UMAP) plug-in in FlowJo v. 10.6.1 and standard settings.
Techniques: Fluorescence, Microscopy, Isolation, Injection, Labeling
